Repair and enumeration of injured coliforms by a plating procedure.
نویسندگان
چکیده
Surface plating of coliforms on Trypticase soy agar, followed by 1 to 2 h of incubation at 25 C and subsequent overlay with violet red bile agar, was found to be a useful method for the repair and enumeration of coliforms injured by freezing.
منابع مشابه
Repair and enumeration of injured coliforms in frozen foods.
Two strains of Escherichia coli manifested death and repairable injury after being frozen in water or sterile foods at -20 C. The injured survivors were inhibited from forming colonies on violet red bile agar (VRBA) or deoxycholate lactose agar; this inhibition was greater when enumeration was done by the pour plate method instead of the surface or surface-overlay method. Injured cells repaired...
متن کاملPlating procedure for the enumeration of coliforms from dairy products.
A "repair-detection" procedure consisting of pour plating of food samples with Trypticase soy agar, followed by 1-h repair incubation at room temperature and subsequent overlay with violet red bile agar, was found to be an effective method for the detection of injuried and uninjuried coliforms from dairy products. This method was relatively less effective for the detection of coliforms in many ...
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AIMS A rapid and simple method for enumerating uninjured and sublethally injured bacterial cells, the twofold dilution method (2FD), was developed and evaluated. METHODS AND RESULTS Following twofold serial dilution of samples in a 96 well microtiter plate, double strength selective broth or nonselective broth was added to each well. For resuscitation of heat-injured (55 degrees C for 10 min)...
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The objective of this study was to compare conventional plating to SimPlate methods for enumeration of aerobic microorganisms, coliforms and Escherichia coli in samples from farm animals and their environment. Samples evaluated included rectal swabs, fecal material, bedding, litter and feed and soil from beef and dairy cattle, swine and poultry farms. Standard method agar pour plating and conve...
متن کاملAgar underlay method for recovery of sublethally heat-injured bacteria.
A method of recovering sublethally heat-injured bacteria was developed. The procedure (termed the agar underlay method) uses a nonselective agar underlaid with a selective medium. In a two-chambered petri dish, the Lutri plate (LP), a nonselective agar is inoculated with a population of sublethally heat-injured bacteria. After a 2-h repair incubation period, selective agar is added to the botto...
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ورودعنوان ژورنال:
- Applied microbiology
دوره 29 4 شماره
صفحات -
تاریخ انتشار 1975